CO1 gene: barcoding and diseases
Acquiring highly detailed taxonomic data is considered to be difficult, as identification established through observations of morphological attributes suffers from issues such as cryptic species, polymorphisms, small size and damaged specimens (Pyšek et al., 2013). Barcoding of DNA has facilitated taxonomic identification, and can be routinely utilised in bioassessment (Eric et al., 2014). The barcoding method is used to identify taxa aligning sequences of DNA from a normal genetic locus, such as the mitochondrial gene cytochrome c oxidase subunit I (COI) and comparing them with other sequences. These genes can also be used for phylogeographic investigation, and to understand the genetic lineage of species with the aid of molecular biology techniques.
Why is CO1 gene is a good gene?
Low number of insertions and deletions
Fast mutation rate (affects the process of speciation)
DNA extractions PCR Sanger sequencing NGS