CO1 gene: barcoding and diseases

CO1 gene

Title

Intro:

CO1 barcoding

Acquiring highly detailed taxonomic data is considered to be difficult, as identification established through observations of morphological attributes suffers from issues such as cryptic species, polymorphisms, small size and damaged specimens (Pyšek et al., 2013). Barcoding of DNA has facilitated taxonomic identification, and can be routinely utilised in bioassessment (Eric et al., 2014). The barcoding method is used to identify taxa aligning sequences of DNA from a normal genetic locus, such as the mitochondrial gene cytochrome c oxidase subunit I (COI) and comparing them with other sequences. These genes can also be used for phylogeographic investigation, and to understand the genetic lineage of species with the aid of molecular biology techniques.

Why is CO1 gene is a good gene?

Highly conserved

No introns

Low number of insertions and deletions

Large database

Fast mutation rate (affects the process of speciation)

Techniques:

DNA extractions   PCR   Sanger sequencing NGS

Discussion

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Conclusion

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